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乙醇酸氧化酶活性檢測試劑盒
更新時間:2024-06-26
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Glycolate Oxidase (GO) Activity Assay Kit
乙醇酸氧化酶活性檢測試劑盒
檢測樣本量:50 Samples
主要檢測設(shè)備及配套:紫外分光光度計/1 mL石英比色皿(d=10 mm)
預(yù)計測定時間:4 h (50 Samples)
試劑儲存條件:按照標(biāo)簽要求儲存
檢測方法: 乙醇酸比色法
Glycolate Oxidase (GO) Activity Assay Kit
乙醇酸氧化酶活性檢測試劑盒
①若A1測定大于0.9,建議將粗酶液使用提取液適當(dāng)稀釋后再測定,計算時相應(yīng)修改;
②反應(yīng)體系中底物易發(fā)生自氧化,設(shè)置空白組作為試劑質(zhì)量檢測孔,正常情況下?A空白應(yīng)小于0.02,試驗前可測定空白組以確定底物狀態(tài);
③準(zhǔn)確在10 s和130 s處完成讀數(shù),以保證實驗結(jié)果的準(zhǔn)確性;
[1]Zhong Y, Xu J, Zhao X, Qu T, Guan C, Hou C, Tang X, Wang Y. Balancing Damage via Non-Photochemical Quenching, Phenolic Compounds and Photorespiration in Ulva prolifera Induced by Low-Dose and Short-Term UV-B Radiation. International Journal of Molecular Sciences. 2022; 23(5):2693. (IF=5.923)